missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Assays
  5. Class-Specific Protein Assay Kits
  6. 10677914

Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit

Product Code. 10677914 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
500 mg
Unit Size:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10677914 500 mg 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10677914 Supplier Thermo Scientific™ Supplier No. 23260

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Beschreibung

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).

Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

  • Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars

  • Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards

  • Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols

  • Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

TRUSTED_SUSTAINABILITY

Spezifikation

Content And Storage Sufficient For: 60 tube assays or 250 microplate assays
• Sodium meta-Periodate, 500 mg
• Pierce Glycoprotein Detection Reagent, 500 mg
• Glycoprotein Assay Buffer, 250 mL
• Glycoprotein Standards Set, 5-protein set
Description Pierce Glycoprotein Carbohydrate Estimation Kit
Assay Glycoprotein Carbohydrate Estimation
Detection Method Colorimetric
Product Line Pierce
Product Type Glycoprotein Carbohydrate Estimation
Specificity Not Target-Specific
Sufficient For 60 Tube Assays or 250 Microplate Assays
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 500 mg
Mehr anzeigen Weniger anzeigen

For Research Use Only. Not for use in diagnostic procedures.

Name des Produkts
Wählen Sie ein Thema

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.