Thermo Scientific™ Pierce™ Protein L Magnetic Beads

Features of Protein L Magnetic Beads:

• Selective—immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
• Low non-specific binding—stable, pre-blocked beads provide clean purification of antibody
• Versatile—beads are compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher Instruments)

Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains. The beads are commonly used to purify monoclonal antibodies in cell culture supernatants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput workflows.

Applications:

• Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads
• Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L IP and co-IP assays
• Purification of ScFv and Fab fragments containing kappa light chains

Thermo Scientific Pierce Protein L Magnetic Beads are typically used for purifying mouse and human antibodies containing kappa light chains from serum, cell culture supernatant or ascites. Protein L can bind a broader range of Ig classes than Protein A or Protein G, including IgG, IgM, IgA, IgE and IgD. Protein L binds strongly to human (kappa I, III and IV only), mouse (kappa I only), rat and pig immunoglobulins. It binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep immunoglobulins. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. The protocol for the Pierce Protein L beads has been optimized to allow for high recovery and high purity of isolated antibodies.