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Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit
Tous les produits Thermo Scientific ProduitsDescription
Thermo Scientific™ Pierce RNA 3' End Biotinylation Kit is optimized for labeling the 3-prime terminal end of RNA probes to facilitate their use as probes or targets in EMSA and other methods for studying protein-RNA interactions.
Highlights:
Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems
Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing
Easy to use – RNA ligase and optimized reaction buffer are included
Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes
End-labeled – results in minimal disturbance of RNA secondary structure
Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides
Spécification
Spécification
| Content And Storage | Sufficient For: 20 labeling reactions (each with 50 pmol RNA) • T4 RNA Ligase Reaction Buffer (10X), 100 μL • T4 RNA Ligase (20,000 U/mL), 40 μL • Non-labeled RNA Control (10 μM), 100 μL • Biotinylated IRE RNA Control (125 nM), 35 μL • RNase Inhibitor (40 U/μL), 2 × 10 μL • Nuclease-free Water, 1.5 mL • DMSO, 200 μL • 30% PEG, 300 μL • Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL • Glycogen (20 mg/mL), 20 μL Store all components at -20°C |
| Format | Kit |
| Product Line | Pierce |
| Product Type | RNA Labeling Kit |
| Quantity | 20 Reactions |
For Research Use Only. Not for use in diagnostic procedures.
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