missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Gel Electrophoresis Equipment and Supplies
  4. Gel Electrophoresis Reagents and Buffers
  5. Gel Electrophoresis Buffers and Diluents
  6. 10617514

Thermo Scientific™ Pierce™ SDS-PAGE Sample Prep Kit

Product Code. 10617514 Shop All Thermo Scientific Products
Click to view available options
Quantity:
50 reactions
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10617514

Brand: Thermo Scientific™ 89888

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Quickly and easily remove high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis.

Thermo Scientific™ Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Highlights:

  • Eliminates gel artifacts caused by incompatible contaminants – removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis

  • Compatible with the BCA protein assay – allows protein quantitation of the processed sample before gel-loading

  • Enriches dilute protein solutions – concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes

  • Powerful and flexible – process 2 to 350μL of sample containing up to 70μg of protein; recover 75 to 85% of the total protein in 50μL of elution buffer

  • Convenient, complete kit – includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

    • Includes:

      PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Upon receipt store kit at room temperature.
Buffer Sample Loading Buffers
Gel Type SDS-PAGE
Product Type SDS-PAGE Sample Prep Kit
Gel Compatibility SDS-PAGE Gels
Product Line Pierce
Quantity 50 reactions

Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • Pierce SDS-PAGE Sample Prep Kit
 Signal Word
  • Danger
 Hazard Category
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H351-Suspected of causing cancer.
  • H373-May cause damage to organs.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P271-Use only outdoors or in a well-ventilated area.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P314-Get medical advice/attention if you feel unwell.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Kieselguhr, calcined

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.