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Thermo Scientific™ Stop Solution for TMB Substrates

Product Code. 10187433 Shop All Thermo Scientific Products
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Quantity:
55 mL
Unit Size:
55mL
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10187433 55 mL 55mL
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Product Code. 10187433 Supplier Thermo Scientific™ Supplier No. N600

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Description

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

TMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5«-tetramethylbenzidine (TMB). Addition of sulfuric acid stop solution changes the color from blue to yellow, stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

  • Size: 55mL

  • Sufficient For: five 96-well microplates

  • Application: ELISA

Instructions: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 to 100μL per well). Use at full strength.
TRUSTED_SUSTAINABILITY

Specifications

Product Type Stop Solution
Quantity 55 mL
Form Liquid
For Use With (Application) ELISA
Content And Storage Store at 2–8°C.

Safety and Handling

compliance-icons
Product Identifier
  • Stop Solution
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
 Hazard Statement
  • H332-Harmful if inhaled.
 Precautionary Statement
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P310-Immediately call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contains : sulphuric acid

For Research Use Only. Not for use in diagnostic procedures.

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