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Description
Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.
Highlights:
Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF
Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins
Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature
Includes:
Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL
Spécification
Spécification
| Product Type | Western Blot Enhancer |
| Detection Method | Colorimetric, Chemiluminescence |
| Form | Liquid |
| For Use With (Application) | Western Blot |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | Pierce |
| Quantity | 500 mL |
| Content And Storage | Upon receipt store kit at room temperature. |
For Research Use Only. Not for use in diagnostic procedures.
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