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Invitrogen™ Platinum™ Tfi DNA Polymerase
Beschreibung
Platinum™ Tfi DNA Polymerase combines Platinum™ automated hot-start technology with a non-Taq, surfactant-free PCR enzyme derived from Thermus filiformis. PCR performance is comparable to Platinum™ Taq DNA polymerase in yield, specificity, fidelity, and robustness for targets less than 1 kb in length. In addition, Platinum™ Tfi is available in a 5'Exo (-) form for PCR applications that do not require the polymerase to have 5'exonuclease activity. With Platinum™ Tfi, you'll:
- Improve PCR sensitivity and yield with an automated hot start
- Generate more available enzyme during the enzyme activation step for improved PCR robustness
- Amplify more DNA with less enzyme per reaction
Available for OEM:
Unlike Platinum™ Taq DNA Polymerase, a customized version of Platinum™ Tfi DNA Polymerase can be made available for resale as a part of a PCR kit for basic research, diagnostics, or other applied fields. Please contact an Invitrogen representative for more information.
Applications:
Amplification of DNA from complex genomic, viral, and plasmid templates.
Unit definition:
One unit of Platinum™ Tfi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into DNA in 30 minutes at 74°C.
Contents:
Platinum™ Tfi DNA Polymerase is supplied with a magnesium free 5X Platinum™ Tfi Reaction Buffer and a separate vial 50 mM MgCl2.
Contents and Storage:
Platinum™ Tfi DNA Polymerase is supplied with 5X Platinum™ Tfi Reaction Buffer and 50 mM Magnesium Chloride. Store at -20°C (non-frost-free).
Spezifikation
Spezifikation
| Content And Storage | Store in freezer (-5 to -30°C). |
| GC-Rich PCR Performance | Low |
| Polymerase | Tfi DNA Polymerase |
| Reaction Speed | Standard |
| Exonuclease Activity | 5' - 3' |
| Product Line | Platinum |
| Quantity | 500 unit |
| Shipping Condition | Dry Ice |
| Fidelity (vs. Taq) | 1 X |
| Hot Start | Built-In Hot Start |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
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