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Invitrogen™ Platinum™ Tfi Exo(-) DNA Polymerase

Product Code. 10240043
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Product Code. 10240043 Supplier Invitrogen™ Supplier No. 60684050

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Description

Platinum™ Tfi Exo(-) DNA Polymerase

Platinum™ Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

Applications
Platinum™ Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum™ Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum™ Taq in yield, specificity, fidelity, and robustness. Like Platinum™ Taq, Platinum™ Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.
TRUSTED_SUSTAINABILITY

Specifications

Concentration 5X
Content And Storage Platinum™ Tfi DNA Polymerase (500 units total, at 5U/μL) is supplied with 5X Platinum™ Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).
GC-Rich PCR Performance Low
Polymerase Tfi DNA Polymerase
Reaction Speed Standard
Product Line Platinum
Product Type DNA Polymerase
Quantity 500 units
Shipping Condition Dry Ice
Fidelity (vs. Taq) 1 X
Hot Start Built-In Hot Start
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 1 kb or less
Volume 5.2 mL
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For Research Use Only. Not for use in diagnostic procedures.

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