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Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Description
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.
Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.
Features
- Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
- Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
- Same performance as Platinum Taq DNA Polymerase manufactured using conventional process
Applications
- Amplification of DNA from complex genomic and viral templates
- Hot start PCR
- RT-PCR
Specifications
Specifications
| Concentration | 5 U/μL |
| Content And Storage | • Platinum Taq DNA Polymerase, DNA-free, 100 μL • 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL • 50 mM MgCl2, DNA-free (1 mL) Store at -20°C in a non-frost-free freezer. |
| Format | Tube |
| GC-Rich PCR Performance | Low |
| Polymerase | Platinum Taq DNA Polymerase |
| Reaction Speed | Standard |
| Product Type | Taq DNA Polymerase |
| Quantity | 500 Units |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Hot-start PCR |
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For Research Use Only. Not for use in diagnostic procedures.
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