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Thermo Scientific™ PNPP Substrates
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Description
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.
Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
The substrate is offered in several formats:
PNPP Substrate
• Powder Form
PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance
PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.
1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.
Specifications
Specifications
| Description | PNPP Substrate Kit |
| Product Type | ELISA Substrate |
| Quantity | 105 tablets kit |
| Detection Method | Colorimetric |
| Format | Kit |
| Product Line | Pierce™ |
| Substrate Properties | Chemical Substrate |
| Substrate Type | Alkaline Phosphatase |
| Content And Storage | Sufficient for: 5 mL of typical 1X substrate solution per tablet (525 mL total, approx. 5250 wells at 100 μL per well) • Diethanolamine Buffer, 225 mL • PNPP Tablets, 105 tablets (5 mg/tablet) Store buffer at 4°C and PNPP tablets at -20°C. |
Safety and Handling
- PHOSPHATE SUBSTRATE KITNPP
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H373-May cause damage to organs.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P314-Get medical advice/attention if you feel unwell.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Diethanolamine, Sodium chloride, Phosphoric acid, 2,2'-iminodiethanol
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