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Gibco™ pOptiVEC™-TOPO™ TA Cloning™ Kit

Product Code. 10317963
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Product Code. 10317963 Supplier Gibco™ Supplier No. 12744017

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Description

Express Kit is designed for efficient growth and transfection of dihydrofolate reductase- deficient (DHFR) Chinese hamster ovary (CHO) DG44 cells in suspension culture

  • The OptiCHO™ Express Kit is designed for efficient growth and transfection of dihydrofolate reductase- deficient (DHFR) Chinese hamster ovary (CHO) DG44 cells in suspension culture.
  • The kit provides a complete workflow protocol as well as the necessary components for efficient transfection of DHFR DG44 cells in a serum-free environment and subsequent stable cell line development, including:
  • pOptiVEC™-TOPO™ vector - a bicistronic cloning vector containing a DHFR gene for stable cell line development in CHO-DG44 cells (Figure 1); the vector is TOPO™ adapted and enables cloning of a PCR product containing your gene of interest with >85% efficiency
  • FreeStyle™ MAX Transfection Reagent - an animal origin-free transfection reagent offering high transfection efficiencies and consistent results
  • CD OptiCHO™ Medium - a chemically defined, protein-free medium (without hypoxanthine or thymidine) specifically designed for high-density suspension cell culture of recombinant CHO-DG44 cells and secreted protein yield
  • CD DG44 Medium - a chemically defined, protein-free medium supplemented with hypoxanthine and thymidine to support optimal growth of DG44 cells in suspension culture
  • DG44 cells - preadapted to CD DG44 medium and selected for superior cell growth and transfection efficiencies
  • In addition, the OptiCHO™ Express Kit provides GIBCO™ OptiPRO™ SFM for improved DNA-lipid complex formation, L-glutamine (provided separately for increased media stability), and Pluronic™ F-68 to control shear forces in suspension cultures.
  • Storage: Store Pluronic™ F-68 at 15°C to 30°C. Store CD DG44 Medium, OptiPRO™ SFM, CD OptiCHO™ Medium, and FreeStyle™ MAX Transfection Reagent at 2°C to 8°C in the dark. Store L-glutamine, pOptiVEC™-TOPO™ vector, CMV Forward Sequencing Primer, and EMCV IRES Reverse Sequencing Primer at -5°C to -20°C. Store One Shot™ TOP10 Chemically Competent E. coli cells at -80°C. Store DG44 cells in liquid nitrogen.
TRUSTED_SUSTAINABILITY

Specifications

Cloning Method TOPO™-TA
Constitutive or Inducible System Constitutive
Delivery Type Transfection
Format Kit
For Use With (Application) Cloning
Key Functions Protein Production, Stable Cell Line Development
Product Line Gibco
Product Type Cloning Kit
Promoter CMV
Protein Tag Untagged
Quantity 1 Kit
Selection Agent (Eukaryotic) HT (-) Media, MTX (Methotrexate)
Vector TOPO-TA Vectors
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For Research Use Only. Not for use in diagnostic procedures.

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