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Thermo Scientific™ POROS™ CaptureSelect™ IgM-XL Affinity Matrix

Product Code. 30245355 Shop All Thermo Scientific Products
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Product Code. 30245355 Supplier Thermo Scientific™ Supplier No. 2812892005
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Thermo Scientific™ POROS™ CaptureSelect™ IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.

POROS CaptureSelect IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.

Features of this affinity matrix include:

  • IgM purification from plasma and serum
  • Purification of monoclonal IgM from cell cultures
  • Efficient purification of IgM with high yields and retained activity
  • Excellent scalability
  • Non-animal derived
  • High flow rates
  • Cross-reactivity towards mouse and rat IgM

POROS CaptureSelect IgM-XL Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using this affinity matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (animal origin-free).

Main characteristics

  • Matrix: cross-linked poly(styrene-divinylbenzene)
  • Average particle size: 50 μm
  • Ligand: POROS CaptureSelect IgM-XL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >5 g/L resin (determined with polyclonal human IgM from serum)
  • Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
  • Mechanical resistance: 100 bar (1450 psi, 10 MPa)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Matrix Cross-linked poly(styrene-divinylbenzene) POROS beads
Content And Storage Store at 2°C to 8°C in a light-protected environment. Avoid direct exposure to sunlight. Do not freeze
Max. Operating Pressure 100 bar
Shipping Condition Wet Ice
Particle Size 50 μm
Form Resin Slurry
Product Line POROS CaptureSelect
Quantity 5 mL
Type Affinity Matrix
Ligand Type CaptureSelect IgM-XL Affinity Ligand
Stationary Phase 20% Ethanol
Binding Capacity >5 g/L
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