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Invitrogen™ Power Blotter 1-Step™ Transfer Buffer (5X)

Product Code. 15816172 Shop All Thermo Scientific Products
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Product Code. 15816172 Supplier Invitrogen™ Supplier No. PB7300

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Description

Invitrogen Power Blotter 1-Step Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter System.

Invitrogen Power Blotter 1-Step Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter System. Transfer is compatible with commonly used detection methods such as staining, chemiluminescence, or fluorescence.

Features of Power Blotter 1-Step Transfer Buffer:
• Fast—high ionic strength formulation allows for 5- to 12-minute protein transfer when used with compatible fast semi-dry blotting systems
• Optimized—designed for seamless and reliable performance with the Power Blotter System
• Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply
• Economical—no need for special or costly consumables that are required by other fast semi-dry transfer devices

When used with the Power Blotter System, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 12 minutes with efficiency that is similar to conventional western blotting techniques. Fast blotting methods require a high-current power supply, such as the Power Blotter System, and an optimized high ionic strength transfer buffer, such as Power Blotter 1-Step Transfer Buffer.

By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (∼22–23 mA/cm2) and voltage is limited to 25 V.

Power Blotter 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size (Cat. No. PB3310)
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini (PB3210)
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size (PB5310)
Power Blotter Pre-cut Membranes and Filters, PVDF, mini (PB4210)

TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Mode of Transfer Semi-Dry
Product Line 1-Step
Type Transfer Buffer
Quantity 1 L
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

What is the difference between the Power Blotter System and the Power Blotter XL System?

The Power Blotter System (Cat. No. PB0012) comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter Cassette (Cat. No. PB0002). The Power Blotter System can transfer one midi or up to two mini gels at the same time. The Power Blotter XL System (Cat. No. PB0013) on the other hand comes with the Power Blotter Station (Cat. No. PB0010) plus Power Blotter XL Cassette (Cat. No. PB0003). The Power Blotter XL System can transfer up to two midi or four mini gels at the same time.
Is it possible to do gel staining with the Power Blotter System/Power Blotter XL System?

Even though the Power Blotter System/Power Blotter XL System does not have the staining program, this can be introduced. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.
What is the material used for the anode and cathode plates of the Power Blotter Cassette/Power Blotter Cassette XL?

The top (cathode) is stainless steel and the bottom (anode) is platinum-coated stainless steel.

The central European or UK power cord for the Power Blotter System/Power Blotter XL System does not work in Switzerland. Do you offer a Swiss power cord?

Yes, you may purchase the Universal Cable Kit (Cat. No. 4377117).
I have lost the power cord for the Power Blotter Station. Is it sold as a stand-alone item?

Here are the SKUs for the power cord. Please order the one appropriate for your region:

- Cat. No. 84858: Power Cord with C13 Connector, North America
- Cat. No. 84859: Power Cord with C13 Connector, Continental Europe and South Korea
- Cat. No. 84860: Power Cord with C13 Connector, United Kingdom and Singapore
- Cat. No. 84855: Chinese 10 Amp Cordset w/ C13 Connector
- Cat. No. 84856: Japanese 15 Amp Cordset w/ C13 Connector
- Cat. No. 84857: Australian 10 Amp Cordset w/ C13 Connector
Are Power Blotter Select Transfer Stacks compatible with immunofluorescence detection?

Yes, Power Blotter Select Transfer Stacks are compatible with immunofluorescence detection.
Can I use PVDF and nitrocellulose membranes for transfer of gels using the Power Blotter System/Power Blotter XL System?

Yes, both PVDF and nitrocellulose membranes are compatible with the Power Blotter System/Power Blotter XL System. You can use 0.2 µm and 0.45 µm pore size membranes. Our Power Blotter Pre-cut Membranes and Filters (PVDF and nitrocellulose) come with 0.45 µm pore size membranes. Our Power Blotter Select Transfer Stacks (PVDF and nitrocellulose) come with 0.2 µm pore size membranes.
The Power Blotter Cassette/Power Blotter Cassette XL is difficult to open. What can I do?

This may be due to salt deposited on moving parts inside the cassette. We recommend rinsing the cassette top and bottom under warm water while removing any sticky salt residue with a gloved hand. Briefly rinse with deionized water and place in a rack to dry. For more thorough cleaning, immerse the unassembled cassette in warm water and use a gloved hand or clean sponge to remove any sticky salt residue. Rinse with deionized water and place perpendicular in a rack to dry.
Note: Failure to keep the cassette top (cathode) and bottom (anode) clean will result in the sticking of moving parts and lead to poor transfer efficiency.
Can I transfer EMSA gels using the Power Blotter System/Power Blotter XL System?

Yes. You may use the same method that was used with the G2 Fast Blotter: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/transfer-emsa-gels-using-g2-fast-blotter.html

I have used the G2 Fast Blotter in the past. Can I apply my standardized method to the Power Blotter System/Power Blotter XL System? Is there a need to do revalidation?

No, revalidation is not necessary. The firmware is the same and the blotting efficiency is the same.

Can I use my own consumables for standard and fast semi-dry transfer with the Power Blotter System/Power Blotter XL System?

You can use your own consumables (membrane, filter paper with 2.48 mm thickness, and buffer) for standard semi-dry transfer on the Power Blotter System/Power Blotter XL System following the parameters recommended on page 20 of the Power Blotter System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017053-PowerBlotterSystem-UG.pdf).

For fast semi-dry transfer on the Power Blotter System/Power Blotter XL System, you can use your own membrane and filter paper (with 1.8 mm thickness), however, the Power Blotter 1-Step Transfer Buffer or the Pierce 1-Step Transfer Buffer would need to be used.

I want to prepare my own transfer stack for use with the Power Blotter System/Power Blotter XL System. Can I use any filter paper?

Yes, but you need to ensure that the total thickness of the filter paper stack on either side of the gel/membrane does not exceed 1.8 mm.
Does the Power Blotter System/Power Blotter XL System allow fast transfer?

Yes, utilization of the Power Blotter 1-Step Transfer Buffer (5X) allows fast transfer (within 5 min for low MW proteins/within 7 min for mixed range MW proteins/within 10 min for high MW proteins). The Power Blotter can also be used with the Pierce 1-Step Transfer Buffer (Cat. No. 84731, 84731X5, or 84742) for fast transfer.
Can I use the Power Blotter System/Power Blotter XL System with my own homemade transfer buffer?

Yes, the Power Blotter System/Power Blotter XL System is an open system and you can use your own transfer buffer. However, keep in mind that your own transfer buffer will result in a prolonged transfer time (˜60 min), whereas a fast transfer (5-10 min) can be achieved using the Power Blotter 1-Step Transfer Buffer (5X) or the Pierce 1-Step Transfer Buffer.
I have a G2 Fast Blotter but my cassette broke. Do you sell a spare cassette?

Yes, you can order the Power Blotter Cassette XL (Cat. No. PB0003).
Note: The Power Blotter Cassette (Cat. No. PB0002) is not compatible with the G2 Fast Blotter.
What is the difference between the Power Blotter Cassette (Cat. No. PB0002) and the Power Blotter Cassette XL (Cat. No. PB0003)?

The Power Blotter Cassette (Cat. No. PB0002) is designed to blot 1-2 mini gels at a time or 1 midi gel and the Power Blotter Cassette XL (Cat. No. PB0003) is designed to blot 1-4 mini gels or 1-2 midi gels at a time. Both cassettes can be used with the Power Blotter. On the other hand, the traditional G2 Fast Blotter is only compatible with the Power Blotter Cassette XL (Cat. No. PB0003); the Power Blotter Cassette (Cat. No. PB0002) will not work with the G2 Fast Blotter.

The power station on my G2 Fast Blotter has broken. How can I replace the station?

You can purchase the Power Blotter Station (Cat. No. PB0010) and the G2 Fast Blotter cassette is compatible with it. The new Power Blotter System is designed for Western transfer alone and does not have a staining module; therefore, the G2 Fast Blotter staining cassette cannot be used on the Power Blotter Station.
Do you offer a staining cassette for the Power Blotter System/Power Blotter XL System?

We do not offer a staining cassette for the Power Blotter System/Power Blotter XL System but it can be programmed to stain gels. Please see the user guide for our Pierce Mini/Midi Gel Power Staining Kit (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017799_22839_22840_powerstain_midi_minigel_UG.pdf) for how to create a Custom Staining Method.
Is there a difference in transfer efficiency when blotting 1 or 2 mini gels at a time using the Power Blotter System, and 1, 2, or 4 mini gels at a time using the Power Blotter XL System?

No, 1-2 mini gels and 1, 2, or 4 mini gels can be transferred at the same time with the same transfer efficiency using the Power Blotter System and Power Blotter XL System, respectively.

For Research Use Only. Not for use in diagnostic procedures.

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