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Invitrogen™ pPIC6α A, B, & C Pichia Vectors
Description
pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection, high-level expression, and rapid protein purification and detection. The pPIC6 A, B, & C vectors are designed for high-level secreted expression.
Both sets of vectors have the following features:
- Blasticidin resistance for direct selection of multi-copy integrants
- Inducible AOX1 promoter for high-level expression in Pichia pastoris
- C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
- C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating
- resin and detection with an Anti-His(C-term) Antibody
- pPIC6 also features:
- An É-factor secretion signal for efficient transport of proteins to the medium
Specifications
Specifications
| Promoter | AOX1 |
| Product Type | Pichia Expression Vector |
| Content And Storage | Each kit includes: • Expression vector in A, B, & C versions, 20 μg/vector (store at -20°C) • lacZ (intracellular) or HSA (secreted) control vector, 20 μg (-20°C) • X-33 Pichia stab (4°C) The Starter Kits also include 50 mg of Blasticidin, store at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Protein Tag | His Tag (6x), c-Myc Epitope Tag |
| Quantity | 20 μg |
| Vector | pPIC |
For Research Use Only. Not for use in diagnostic procedures.
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