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Invitrogen™ Predictor™ hERG Fluorescence Polarization Assay Kit

Product Code. 10532304
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Product Code. 10532304 Supplier Invitrogen™ Supplier No. PV5365
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Description

Predictor™ hERG Fluorescence Polarization Assay Kit

The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.

TRUSTED_SUSTAINABILITY

Specifications

Color Red
Conjugate Predictor hERG Tracer Red
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
Quantity 1 Kit
Packaging 384-well Plate
Content And Storage

PV5365 Predictor™ hERG Fluorescence Polarization Assay Kit
(Other materials are required separately; please refer to the protocol)
Includes:
• Predictor™ hERG Membranes
• Predictor™ hERG Tracer Red
• Predictor™ hERG Buffers
• E-4031
• Predictor™ hERG Fluorescence Polarization Assay Protocol
• Certificate of Analysis
Store the Predictor™ hERG Membranes at -80°C. The Predictor™ Tracer Red, Predictor™buffers, and E-4301 should be stored at -20°C.
Assay Entry Biochemical binding
Druggable Target Ion Channels
Gene Symbol hERG
Readout End Point
Shipping Condition Dry Ice
System Type Predictor™ System
Target Entry hERG
Product Line Predictor
Protein Family Ion Channels
Product Type Assay Kit
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For Research Use Only. Not for use in diagnostic procedures.

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