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Description
Prepacked with Protein A Sepharose™ High Performance Media for coupling of antibodies of IgG subclasses; used with a standard microcentrifuge
- Fast binding kinetics and high capacity provide high yield
- Classic (antigen eluted with antibody) and cross-link (antigen eluted separately) protocols provide flexibility
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows
- Easy scale-up with HiTrap Protein A HP prepacked columns
- Additional protocol for small-scale purificaiton of monoclonal and polyclonal antibodies from serum and cell culture supernatants
Specifications
Specifications
| Binding Capacity | >1 mg Human IgG well |
| For Use With (Application) | Centrifugation |
| Sample Volume | 600 μl |
| For Use With (Equipment) | Standard microcentrifuge. |
| Content And Storage | 2°C to 30°C, 20% Ethanol |
| Quantity | 16 Columns |
Safety and Handling
Product Identifier
- Protein A HP SpinTrap
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: ethanol
Product Title
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