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  5. Invitrogen™ Quant-iT™ RNA Assay Kits and Quant-iT RNA HS Reagent 

Invitrogen™ Quant-iT™ RNA Assay Kits and Quant-iT RNA HS Reagent

Product Code. 10174582
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Quantity:
1 kit
1,000 assays
1000 assays
Assay:
RNA Quantitation
RNA Quantitation, BR
RNA Quantitation, ext range
Quantitation Range:
20 to 1000 ng
200 to 10,000 ng
5 to 100 ng
Unit Size:
1 set
Each
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Product Code. Quantity Assay Quantitation Range unitSize
10174582 1 kit RNA Quantitation 5 to 100 ng 1 set
10582894 1,000 assays RNA Quantitation, BR 20 to 1000 ng Each
15888957 1000 assays RNA Quantitation, ext range 200 to 10,000 ng Each
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Product Code. 10174582 Supplier Invitrogen™ Supplier No. Q33140

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Description

Achieve highly selective and accurate quantification of high-abundance RNA samples with the fluorescent Quant-iT RNA Reagent and Assay Kits. These kits are highly selective for RNA over double-stranded DNA, and provide sufficient material for at least 2000 assays in a 96-well microplate format.

Enable easy and accurate RNA quantitation with the Quant-iT RNA Assay Kit, which is also available in broad range (BR) and extended range (XR) configurations, as well as a stand-alone high sensitivity (HS) reagent. These Quant-iT RNA quantification kits are highly selective for RNA over dsDNA and can be used to generate fluorescence-based linear detection ranges of RNA.

Quant-iT RNA Reagent and Assay Kit
The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA.

Quant-iT RNA BR (Broad Range) Assay Kit
The Quant-iT RNA BR (Broad Range) Assay Kit provides an accurate and selective method for quantification of high-abundance RNA samples. While the original Quant-iT RNA kit has great sensitivity, the Quant-iT RNA Broad Range kit is ideal for microarray samples where the concentration of RNA is higher. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 20-1000 ng of RNA.

Quant-iT RNA XR (Extended Range) Assay Kit
The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantification of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 ng/μL to 10,000 ng/μL depending on sample volume, providing a linear detection range of 200–10,000 ng.

For each assay, simply dilute the reagent using the buffer provided, add the sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using a fluorescence-based microplate reader. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well tolerated.

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Assay RNA Quantitation
Detection Method Fluorescence
For Use With (Equipment) Microplate Reader
No. of Reactions 1000 Reactions (200 μL Assay Volume)
Quantitation Range 5 to 100 ng
Product Line Quant-iT
Quantity 1 kit
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT RNA Assay Kit and want to use it with the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application.

Will these Quant-iT RNA kits work for siRNA or microRNA?

No, they are not accurate for such small fragments. The Quant-iT microRNA Assay Kit and Qubit microRNA Assay Kit are good tools for this application.

Can the Quant-iT RNA assay be used to quantitate RNA labeled with digoxigenin?

Yes, the tag does not interfere with the signal.

What are the linear ranges of the Quant-iT RNA kits?

The linear ranges are:

- Quant-iT RiboGreen RNA Assay: 1 ng/mL to 1 µg/mL
- Quant-iT RNA BR Assay: 20-1,000 ng
- Quant-iT RNA HS Assay: 5-100 ng

What is the difference between the Quant-iT RNA assay and the Quant-iT RiboGreen RNA Assay?

There are several differences:

- The Quant-iT RNA Assay uses a far-red dye (excitation 644 nm/emission 673 nm); the Quant-iT RiboGreen RNA assay uses a green fluorescent dye (excitation 500 nm/emission 525 nm).
- The Quant-iT RNA assay is more specific for RNA.
- Quant-iT RNA Assays are less sensitive than the Quant-iT RiboGreen RNA Assay.

Can I make my own assay for the Qubit Fluorometer?

Yes, you can, for Qubit instruments developed after the original Qubit (1.0) Fluorometer. See MyQubit assay instructions here (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-assays/myqubit.html.html).

I have a crude lysate. Will the Quant-iT and Qubit assays work?

Generally, the cleaner the sample the better. Some salts, proteins, and detergents are tolerated in the assays; see the specific assay protocol for which ones and at what concentrations.

How does the accuracy and sensitivity of the Qubit quantitation assays using the Qubit fluorometer compare to a microplate reader?

The accuracy and sensitivity of the Qubit quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.
Can the Qubit kits give an indication of sample quality in nucleic acid samples?

No. The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.
Can I use the Quant-iT and Qubit Kits with other fluorometers?

All Quant-iT and Qubit kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won't have access to the algorithm in the Qubit fluorometer for generating the standard curve provided by the instrument, instead, you must make a few dilutions of the highest standard DNA or RNA (Standard #2) in the Qubit kits to generate a standard curve with multiple data points.
Can I use the original Quant-iT Kits with the Qubit Fluorometer?

No, we do not recommend this. Some of the dyes in the original Quant-iT kits (those NOT listed as “for use with the Qubit fluorometer”) are not compatible with the Qubit Fluorometer. In addition, the new Quant-iT kits (labeled as “for use with the Qubit Fluorometer”) have standards formulated to be compatible with the Qubit fluorometer internal algorithms for the respective assays. The Qubit Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.
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