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R&D Systems™ Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
Description
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
Order Info
The product is shipped at ambient temperature. Upon receipt, store it immediately
Specifications
Specifications
| Accession Number | P01374 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Gene ID (Entrez) | 4049 |
| Molecular Weight (g/mol) | M.W. (Observed): 17-19 kDa, reducing conditions; M.W. (theoretical): 19 kDa |
| Name | Lymphotoxin-alpha/TNF-beta |
| Preparation Method | Reconstitute at 100μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Quantity | 10 μg |
| Source | E. coli-derived human Lymphotoxin-alpha/TNF-beta protein Leu35-Leu205, with an N-terminal Met |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
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