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R&DSystems™ Recombinant Human FGF basic Heat Stable Protein, CF
Description
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.0500-0.600 ng/mL.
Specifications
Specifications
| Accession Number | NP_001997.5 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose. |
| Gene ID (Entrez) | 2247 |
| Molecular Weight (g/mol) | M.W.-observed: 16-19 kDa, under reducing conditions., M.W.-theroretical: 17 kDa |
| Quantity | 500 μg |
| Source | E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288 (with modifications) |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Gene Alias | Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin |
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