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R&D Systems™ Recombinant Mouse CD40 Ligand/TNFSF5 (HA-tag) Protein

Product Code. 18706277
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Quantity:
50 μg
Unit Size:
50µg
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18706277 50 μg 50µg
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Product Code. 18706277 Supplier R&D Systems™ Supplier No. 8230CL050/CF

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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.07-0.35 ng/mL in the presence of a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P27548
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Gene ID (Entrez) 959
Molecular Weight (g/mol) M.W. (Observed): 24-29 kDa, reducing conditions; M.W. (theoretical): 22 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived mouse CD40 Ligand/TNFSF5 protein HA (YPYDVPDYA) GCN4-IZ GGGSGGGSGGGS Mouse CD40 Ligand/TNFSF5 (Met112-Leu260) (N-terminus) Accession # P27548 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Regulatory Status RUO
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Symbol Cd40lg
Specific Reactivity CD40 Ligand/TNFSF5
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity or Quality Grade >95%, by SDS-PAGE with silver staining
Protein CD40 Ligand/TNFSF5
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