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  6. 12739240

Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole

Product Code. 12739240
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Quantity:
50 x 0.75 mL
Unit Size:
Pack of 50
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12739240 50 x 0.75 mL Pack of 50
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Product Code. 12739240 Supplier Thermo Scientific™ Supplier No. R21522

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Description

Determine of the ability of microorganisms, to split indole from tryptophan using BactiDrop™ Kovac's Indole reagent.

Determine of the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule with Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole reagent. Earlier tests to confirm indole production required a xylene extraction procedure to detect small quantities of indole produced from the breakdown of tryptophan. Kovac's utilized amyl alcohol instead of ethyl alcohol to bypass the extraction procedure involved in the Ehrlich-Beohme reagent1. Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of Kovac's reagent2.

Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. Indole combines with dimethyl-aminobenzaldehyde to form a red compound. This is a condensation product formed by an acid splitting of a protein3.Ready-to-use: Liquid reagentEasy to interpret: Positive Test - A pink-red ring between the broth and reagentNegative Test - No color developmentKovac's Indole Reagent contains 5% w/v ρ-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0) and amyl alcohol (CAS 71-41-0) and is supplied as 0.75 mL ampules..Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage 20°C to 25°C
Description BactiDrop Indole, Kovacs
CE Marker Yes
Detectable Analytes Nitrite, Free Nitrogen Gas
Quantity 50 x 0.75 mL

Safety and Handling

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Signal Word
  • Danger
 Hazard Category
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 1 B
  • Serious eye damage/eye irritation Category 1
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H290-May be corrosive to metals.
  • H332-Harmful if inhaled.
  • H314-Causes severe skin burns and eye damage.
  • H335-May cause respiratory irritation.
  • EUH066-Repeated exposure may cause skin dryness or cracking.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
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