missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
Shop All Thermo Scientific Products
Click to view available options
Quantity:
2,500 units
24 x 2,500 units
4 x 2,500 units
Unit Size:
2500 units
Pack of 24
Pack of 4
Beschreibung
RiboLock RNase Inhibitor inhibits the activity of RNases A , B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
- Does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H
- Performs under a wide range of reaction conditions
- Protects RNA from degradation at temperatures up to 55°C
- The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases and ribonucleases confirmed by appropriate quality tests
- Functionally tested in RNA and cDNA synthesis
- E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor
- 49.6kDa monomer Definition of Activity Unit:
- One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%
- Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH 7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [3H]-RNA, 50mg/mL yeast RNA, 8mM DTT
- The enzyme is supplied in: 20mM HEPES-NaOH (pH 7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol
- Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound
- Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min. heating at 70°
Recommended for:
- Inhibition of RNA degradation in the following: in vitrotranscription (1), cDNA synthesis (2), in vitrotranslation (3), isolation of mammalian cell fractions that contain mRNA-protein complex
- RNA purification and storage
- Separation and identification of specific ribonuclease activities
- Studies of tumor suppression
Note:
- DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity
- Recommended concentration 1u/μl of a reaction mixture
Spezifikation
Spezifikation
| Concentration | 40 U/μL |
| Compatible Buffer | Storage Buffer |
| Quantity | 4 x 2,500 units |
| Product Type | RNase Inhibitor |
| Product Line | RiboLock |
For Research Use Only. Not for use in diagnostic procedures.
Berichtigung von Produktinhalten
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.
Name des Produkts
Haben Sie Verbesserungsvorschläge?Übermitteln Sie eine inhaltliche Korrektur