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Gibco™ Sf-900™ III SFM

Sf-900™ III SFM

Brand:  Gibco™ 12658001

Additional Details : Weight : 21.59000kg

Product Code. 15236025

  • 1802.00 € / 20 L
Estimated Shipment: 16-05-2024
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Description

Description

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco™ Sf-900™ III SFM:

  • Superior long-term, high-density growth
  • Optimized for recombinant protein production
  • Serum-free, protein-free, animal origin–free, ready-to-use formulation
  • Improved lot-to-lot consistency

    Superior long-term, high-density growth
    Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

    Optimized for recombinant protein production
    Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

    Serum-free, protein-free, animal origin–free, ready-to-use formulation
    Gibco™ Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

    Improved lot-to-lot consistency
    Gibco™ Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).

    cGMP manufacturing and quality system
    Gibco™ Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
  • Specifications

    Specifications

    2 to 8°C, room temperature
    Insect Cell
    Liquid
    Sterile
    No Phenol Red, No Sodium Bicarbonate, No Sodium Pyruvate
    Cell Culture Grade
    For research use or further manufacturing. Not for use in diagnostic procedures
    Sf-900™ III SFM, 20L, Liquid, Insect Cells Type, Animal Origin-free, Non-CO2 Culture Environment, L-Glutamine, Serum-free, Sterile, Used For Insect Cell Culture, Viral Vaccine Production
    1 X, 1:1 Ratio
    Insect Cell Serum Free Medium (SFM)
    Glutamine
    Cell culture, insect cell culture, bioproduction, viral vaccine production, Sf9 and Sf21 cell culture
    20 L
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    For Research Use Only. Not for use in diagnostic procedures.