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Molecular Probes™ Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 and SYTOX™ Green Dyes, for flow cytometry

Product Code. 10154382
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Product Code. 10154382 Supplier Molecular Probes™ Supplier No. V13240

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Single Channel Dual 384-Well Temperature Verification Kit for PCR 9700 System, 0.02mL

This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to green-fluorescent Alexa Fluor™ 488 dye and dead cells using SYTOX™ Green nucleic acid stain. After treatment with both probes, apoptotic cells show green fluorescence, dead cells show brighter green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished in the FL1 channel of a flow cytometer, freeing the other channels for detection of other fluorescent colors.

View a selection guide for all apoptosis assays for flow cytometry.
TRUSTED_SUSTAINABILITY

Specifications

Quantity 1 kit
Format Tube
Product Type Dead Cell Apoptosis Kit
Content And Storage Contains 1 vial of annexin V, Alexa Fluor™ 488 conjugate (250 μL), 1 vial of SYTOX™ green (100 μL), and 1 bottle of annexin binding buffer (5X solution, 50 mL).

Store in refrigerator (2–8°C) and protect from light.
Flow Cytometer Laser Lines 488
Excitation/Emission SYTOX™ green: 503⁄524, Alexa Fluor™ 488: 499⁄521
No. of Reactions 50
Shipping Condition Wet Ice
Product Line Alexa Fluor, SYTOX
For Use With (Application) Flow Cytometry
Conjugate Alexa Fluor 488, SYTOX Green
For Use With (Equipment) Flow Cytometer
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For Research Use Only. Not for use in diagnostic procedures.

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