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Description
- SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin-columns to isolate without the use of toxic substances such as phenol and chloroform:
- DNA
- RNA
- Protein components from the same sample.
- The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep™ Instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA and proteins are then sequentially purified from the same lysate. Briefly, DNA is adsorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are:
- Precipitated out of solution
- Pelleted down by centrifugation
- Washed and resuspended.
Specifications
Specifications
| Format | Kit |
| Scale | Mini |
| Sample Type | Human, Mouse, Rat |
| Sequencing Type | DNA and RNA Sequencing |
| Quantity | 50 Preps |
Safety and Handling
Product Identifier
- SPINeasy DNA/RNA/Protein All In One Kit, 50 preps
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H373-May cause damage to organs.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P321-Specific treatment (see on this label).
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: Guanidine Hydrochloride, Manganese Sulfate Monohydrate, Calcium Chloride, Zinc Sulfate heptahydrate
Product Title
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