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Description
- Flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows
- Useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin
- Can also be used for the direct enrichment of proteins that are biotinylated. By binding a specific biotinylated protein to the column, protein-protein interactions can also be investigated
- Each well is prepacked with Streptavidin Sepharose™ High Performance Medium
- Easy scale-up to Streptavidin HP SpinTrap™ or HiTrap™ Streptavidin HP prepacked columns
- Can be used with centrifugation or vacuum, manually or in automated robotic systems
Streptavidin Sepharose HP Medium
- Matrix: Highly cross-linked agarose, 6%
- Binding capacity: >300nmol biotin/mL medium; 6mg biotinylated BSA/mL medium
- Ave. particle size: 34μm
- pH Stability (long-term): 4 to 9
- Well volume: 800μL
- Max. sample loading volume: 600μL
- Medium volume: 50μL
Specifications
Specifications
| Binding Capacity | >300nM/mL |
| Flow Rate | <150 cm/hr. |
| Kit Contents | Streptavidin Sepharose HP, spin column and 96-well filter plates filter plates |
| Material | Polypropylene, Polyethylene |
| Support Type | 6% Highly Cross-linked Agarose |
| Content And Storage | 4°C to 8°C |
| Particle Size | 34 μm |
| Quantity | 4 x 96-well Plates |
| For Use With (Application) | Protein enrichment, Protein-Protein interaction investigations |
| Type | Filter Plate |
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Safety and Handling
Product Identifier
- Streptavidin HP MultiTrap
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- MIXTURE LIST-Contains : ethanol
- UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
Product Title
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