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Description
- Can also be used for the direct enrichment of proteins that are biotinylated
- Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows
- Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin
- Each column is packed with Streptavidin Sepharose™ High Performance Medium; for use with a standard microcentrifuge
- Easy scale-up with HiTrap™ Streptavidin HP prepacked columns
Streptavidin Sepharose HP Medium
- Matrix: rigid, highly cross-linked 6% agarose
- Ave. particle size: 34μm
- Binding capacity: >300nmol biotin/mL medium; 6mg biotinylated BSA/mL medium
- pH stability: 4 to 9 (long term)
- Medium volume: 100μL
- Max. sample loading volume: 600μL
Specifications
Specifications
| Binding Capacity | >300nM Biotin/mL |
| Flow Rate | <150 cm/hr. |
| Kit Contents | Streptavidin Sepharose HP, spin column and 96-well filter plates filter plates |
| Material | Polypropylene, Polyethylene |
| Support Type | 6% Highly Cross-linked Agarose |
| Content And Storage | 4°C to 8°C |
| Particle Size | 34 μm |
| Quantity | 16 Columns |
| For Use With (Application) | Gel electrophoresis, liquid chromato graphy |
| Type | SpinTrap Kit |
Safety and Handling
Product Identifier
- streptavidin HP spintrap
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol
Product Title
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