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  5. Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Product Code. 11859290 Shop All Thermo Scientific Products
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Product Code. Quantity unitSize
11859290 100 mL 100mL
10391544 20 mL 20mL
10095983 200 mL 200mL
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Product Code. 11859290

Brand: Thermo Scientific™ 34095

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Description

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

  • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

  • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

  • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

  • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

  • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal
Quantity 100 mL
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Sensitivity Low femtogram-level
Signal Duration 6 to 8 hr.
Sufficient For 12 Mini-Gel Blots, 1000 sq. cm of Membrane
Kit Contents Luminol/Enhancer, 50 mL, Stable Peroxide Buffer, 50 mL
Content And Storage Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store reagents at 4°C.
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For Research Use Only. Not for use in diagnostic procedures.

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