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Thermo Scientific™ Tango™ M1-bla U2OS Cells

Product Code. 10217274
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8 x 106 cells/vial
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10217274 8 x 106 cells/vial Each
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Product Code. 10217274 Supplier Thermo Scientific™ Supplier No. K1861

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Descripción

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Tango™ M1-bla U2OS cells contain the human muscarinic M1 receptor (CHRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ M1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of oxotremorine M. In addition, Tango™ M1-bla U2OS cells have been tested for assay performance under variable conditions.

TRUSTED_SUSTAINABILITY

Especificaciones

Accession Number NM_000738
Assay Entry Cell-based beta-arrestin recruitment
Cell State Dividing Cells
Content And Storage Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Cytoplasmic Tail Native Tail
Detection Method Fluorescence
For Use With (Application) FRET
Human Protein Class G-Protein Coupled Receptor (GPCR)
Population Status Single Cell Clone Origin
Product Line Tango
Quantity 8 x 106 cells/vial
Readout End Point
Reporter Gene BLA (Beta-Lactamase)
Target Entry M1
Type Dividing Cells
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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