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Description
- The Applied Biosystems TaqMan Advanced miRNA Human A and B Cards provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 384-well microfluidic card format. This product contains two cards, enabling accurate quantitation of 754 unique miRNAs in as little as five hours.
- Four TaqMan Advanced miRNA Assay endogenous controls are included in each card to aid in data normalization. Details about the included assays are available in the Target List file. The assays can detect and quantify the mature form of the miRNA from 1–10 ng of total RNA from tissue.
- Required for preparation of the cDNA template that is used with the TaqMan Advanced miRNA assays.
- The kit enables the analysis of: Multiple miRNAs from a single amplified sample, Samples that are limited in quantity, including serum, plasma, or other biological fluids
- Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA assays
- Sensitive—detect as few as 60 copies of input miRNA
- Specific—detect only mature miRNA and distinguish between highly homologous miRNAs
- Small sample input—detect and quantify mature miRNA from as little as 1–10 ng of total RNA or 2 μL of plasma or serum
- Versatile—compatible with tissue and biofluids, including serum and plasma
- TaqMan Advanced miRNA cards provide all the advantages of TaqMan Advanced miRNA assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan Advanced miRNA assays in parallel. With the TaqMan Advanced miRNA cDNA Synthesis Kit, TaqMan Advanced miRNA cards enable a comprehensive expression profile consistent with miRBase v21, providing the ideal miRNA profiling solution.
- TaqMan Advanced miRNA cards are compatible with the following Applied Biosystems qPCR instruments: Viia7, QuantStudio 7 Flex, and QuantStudio 12k Flex.
- TaqMan Advanced miRNA cards require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal RT chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
- These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
Specifications
Specifications
| Content And Storage | Two 384-well arrays The TaqMan miRNA cards are shipped at ambient temperature. Upon arrival, store at 2–8°C and keep protected from light. |
| Detection Method | Primer-probe |
| Format | 384-well microfluidic card |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Fast or Standard |
| Label or Dye | 2 Color |
| Product Line | TaqMan |
| Product Type | Advanced miRNA Human A and B Card |
| Quantity | 754 Reactions |
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Frequently Asked Questions (FAQs)
All materials required for TaqMan® Advanced miRNA Assay workflow using TaqMan® Advanced miRNA Human and B cards (Cat. A31805) can be found on pages 9-11, in the TaqMan® Advanced miRNA Assays User Guide.
Reagents and kits required for the TaqMan® Advanced miRNA Assay workflow include, but are not limited to:
• RNA Isolation Kit (see recommended kits on page 9)
• TaqMan® Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)
• TaqMan® Fast Advanced Master Mix (Cat. No. 4444557)
Please refer to the user guide linked above for a full list of other materials and equipment required for the workflow.
The TaqMan Advanced miRNA array cards allow one to profile over 300 different miRNA targets on one card. Due to the large number of targets and the nature of your sample, it is possible that some miRNA on the card will not be expressed on your sample, or expressed at very low levels. This is one reason why you will not see good amplification plots for every spot on the array card. Another possibility is the sample quality or input. Make sure to check the concentration and quality of the RNA sample before reverse transcription. Check the Ct values for your endogenous control assays on the card, and see if it is in the expected range for your typical samples.
For Research Use Only. Not for use in diagnostic procedures.
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