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Description
Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.
TaqMan MicroRNA Assays are:
- Highly specific—quantitate only mature miRNAs, not precursors
- Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
- Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours
Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.
Availability and sizes
Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.
Species
TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.
Assay Components
TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).
Pre-formulated assay
- Forward primer: 1500 nM
- Reverse primer: 700 nM
- FAM-MGB probe: 200 nM
- RT sequences: 50 nM
TaqMan miRNA Assay selection guide
TaqMan MicroRNA Assays
- Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
- RT chemistry: miRNA-specific RT
- Throughput: Best for 1–10 targets
- Coverage: 205 species available, coverage for miRBase v.21
TaqMan Advanced miRNA Assays
- Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
- RT chemistry: Universal RT
- Throughput: Best for >10 targets
- Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.24
Spécification
Spécification
| Content And Storage | • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size) • 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
| Description | S (50 RT/150 PCR reactions), Inventoried |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Fast |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Product Line | TaqMan |
| Product Type | Configurable Assay |
| Quantity | S (50 RT/150 PCR reactions), Inventoried |
| Afficher plus |
Sécurité et traitement
- TaqMan MicroRNA Assays INV, SM
- Danger
- Carcinogenicity Category 2
- Reproductive toxicity Category 1B
- Specific target organ toxicity after repeated exposure Category 2
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: formamide
For Research Use Only. Not for use in diagnostic procedures.
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