Learn More
Description
A highly specific kit that quantitates only mature miRNAs, not precursors. A sensitive kit that conserves limited samples and requires only 1 to 10 nanograms of total RNA. It is fast, simple and scalable 2-step quantitative RT-PCR assay that provides high-quality results in less than three hours.
Components of this kit are used with the RT primer provided with the TaqMan™ MicroRNA Assay to convert miRNA to cDNA.
Order Info
Shipping Conditions: Dry Ice
Specifications
Specifications
| Concentration | 10X |
| Content And Storage | Contains 10X RT buffer, dNTP mix w/dTTp (100M total), RNase inhibitor (20U/μL), and MultiScribe™ RT enzyme (50U/μL) for 200 reactions. Store at -15 to -25°C. |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR Method | 2-step RT-qPCR |
| Reaction Speed | Fast |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
| Optimal Reaction Temperature | 42°C |
| Product Line | TaqMan |
| Product Type | MicroRNA Reverse Transcription Kit |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.