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Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; Nuclease-Free Water
Ready-to-use qPCR master mixes with premixed UDG optimized for SYBR Green chemistry using standard cycling protocol. Master mixes available with or without blue color.
- UDG in the master mix to prevent carry-over contamination
- Equal performance of blue and colorless master mixes
- Versions with premixed ROX or fluorescein for different real-time platforms
- Standard cycling protocols
- Excellent qPCR performance
- Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
- Sensitivity: single copy detection
- Wide linear range: accurate quantification across 9 orders of magnitude
- Reproducibility and convenience: ready-to-use 2X master mix
Tekniske data
Tekniske data
| Concentration | 2X |
| Content And Storage | Contains: • 4 x 12.5 mL Luminaris HiGreen qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 5000 x 20 μL reactions • 2 x 30 mL Nuclease-Free Water Store at -20°C. |
| Detection Method | SYBR |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene |
| Product Line | Luminaris |
| Product Type | qPCR Master Mix |
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For Research Use Only. Not for use in diagnostic procedures.
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