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  6. Gibco™ TrypLE™ Select Enzyme (10X), no phenol red

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
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Product Code. 10698118
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Product Code. 10698118 Supplier Gibco™ Supplier No. A1217702

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Description

TrypLE™ Select Enzyme (10X), no phenol red

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

  • Gentle on cells—maintains cell health for reproducible results
  • Room-temperature stable—convenient to use
  • Easy to use—substitutes directly into existing protocols, with no inactivation required
  • Animal origin free (AOF)—pure, consistent, and effective



    Gentle on cells
    TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

    Room-temperature stable
    TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

    Easy to use
    TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

    Animal Origin Free (AOF)
    Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

    Product Intended Use
    For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

    cGMP Manufacturing and Quality System
    Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

    Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Chelators EDTA
    Content And Storage Storage conditions: 15–30°C. Protect from light.
    Alternate storage conditions: 2–8°C or -5 to -20°C.
    Shipping conditions: Ambient.
    Shelf life: 24 months from date of manufacture.
    Cell Type Mammalian Cells
    Classification Animal Origin-free
    Concentration 10 X
    Culture Type Adherent Cell Culture
    Form Liquid
    Product Type Cell Culture Dissociation Reagent
    Without Additives No Phenol Red
    Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
    Osmolality 270 - 320 mOsm/kg
    pH 6.9 to 7.4
    Product Line Gibco
    Purity or Quality Grade Cell Culture Grade
    Quantity 500 mL
    Remarks Room Temperature Stable
    Shipping Condition Ambient Temperature
    Source Animal origin-free
    Tested For In vitro Bioassay
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    Frequently Asked Questions (FAQs)

    Can I use TrypLE Select Enzyme to detach cells from glass surfaces?

    Yes, TrypLE Select Enzyme can detach cells from glass surfaces.
    What is the concentration of TrypLE Select Enzyme (10X), no phenol red (Cat. No. A1217701, A1217702, A1217703)?

    TrypLE Select Enzyme (10X), no phenol red (Cat. No. A1217701, A1217702, A1217703) contains 100 µg/mL of the rProtease enzyme.
    How can I assess residual TrypLE reagent remaining after my downstream processing steps?

    We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
    What enzymes do you recommend for passaging mesenchymal stem cells (MSC)?

    We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.

    Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

    There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

    For your TrypLE products, how does the enzyme get inactivated?

    Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

    After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

    Why are my cells not adhering when I transfer them to a new flask?

    Trypsinize this type of cells for a shorter duration or use less trypsin or Gibco TrypLE Select or Gibco TrypLE Express Trypsin Replacement. After trypsinization dilute in 2 to 5 mL of cell culture growth medium transfer the cell suspension to 15 mL centrifuge tube. Centrifuge for 5-10 minutes at 100 x g. Discard the supernatant and suspend cell pellet in 2-5 mL fresh medium. Determine the count and seed the flask according to normal protocol and cell should adhere to the surface.

    Do you have a non-animal-origin enzyme for cell dissociation?

    Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.
    What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

    All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

    The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

    TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

    TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

    CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

    To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

    What are the benefits of Gibco TrypLE reagent over trypsin?

    TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
    What reagents do you offer for cell dissociation, and what are the main differences between them?

    Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

    Do you offer media without phenol red (phenol red-free media)?

    Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
    What can be used to detach cells from plates when coated with a thin layer of collagen I?

    Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

    For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

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