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  7. Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial

Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial

Product Code. 11832133
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No. of Reactions:
200 Reactions
400 Reactions
Unit Size:
200 reactions
400 reactions
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Product Code. No. of Reactions unitSize
11832133 200 Reactions 200 reactions
11842133 400 Reactions 400 reactions
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Product Code. 11832133 Supplier Thermo Scientific™ Supplier No. AB4100A

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Beschreibung

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. This version of the kit comes with a separate vial of ROX reference dye.

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.

  • Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
  • RT and qPCR steps performed in single tube - convenience and reduced contamination risk
  • Use of the entire RT reaction as template for qPCR - improved sensitivity
  • Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg
  • Verso RT enzyme has a wide working temperature range (42° to 57°C) - improved success with GC-rich and other difficult templates
  • Contains an inert blue dye for ease of pipetting during reaction set up
TRUSTED_SUSTAINABILITY

Spezifikation

Content And Storage Contains:
•1 x 50 μL Verso Enzyme Mix
•1 x 250μL RT enhancer
•2 x 1.25 mL 2X 1-Step qPCR Mix
•1 x 25μL ROX, 1mM
Sufficient for 200 x 25 μL reactions.

Store at -20°C
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method 1-step RT-qPCR
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Product Line Verso
Product Type 1-Step RT-qPCR Kit
Quantity 200 reactions
Sufficient For 200 Reactions
For Use With (Application) Gene Expression, Pathogen Detection
No. of Reactions 200 Reactions
Sample Type RNA
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For Research Use Only. Not for use in diagnostic procedures.

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