missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. 17143029

Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

Product Code. 17143029
Change view
Click to view available options
Quantity:
20 Reactions
Unit Size:
20 reactions
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
17143029 20 Reactions 20 reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you
Product Code. 17143029 Supplier Invitrogen™ Supplier No. A11144

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy
Regulatory: Restrictions may apply to the purchase of this product and we may need to request additional documentation from you

Description

The ViraPower HiPerform T-REx Gateway Vector Kit contains the Gateway-adapted ViraPower HiPerform tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells.

The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3/TO/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

  • Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
  • Easy recombination-based cloning using Gateway™ technology
  • Stable, long-term, tetracycline-regulated expression
  • Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features

  • WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
  • Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
  • Blasticidin selection marker for stable selection under control of SV40 promoter

Kit includes

  • pLenti6.3/TO/V5-DEST™ vector
  • pLenti3.3/TR vector
  • pLenti6.3/TO/V5-GW/lacZ plasmid
  • Tetracycline
  • One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
  • pENTRTM Gus positive control plasmid.
TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Inducible
Delivery Type Lentiviral
Inducing Agent Doxycycline, Tetracycline
Promoter CMV/TO
Product Type Vector Kit
Selection Agent (Eukaryotic) Blasticidin
Content And Storage ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg
• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg
• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg
• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.
Store at -20°C unless otherwise noted

One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL
• pUC19 control DNA (10 pg/μL), 50 μL
Store at -80°C

pENTR™ Gus positive control plasmid, store at -20°C.
Antibiotic Resistance Bacterial Ampicillin (AmpR), Blasticidin (BsdR)
Cleavage No Cleavage Site
Gateway Enzyme LR Clonase™ II Plus, LR Clonase™ II
PCR Enzyme Proof-Reading Polymerase
Protein Tag Position (to your gene) C-terminal
Replication Origin pUC Origin
Selection Marker Promoter PGK Promoter
Selection Marker Eukaryotic BsdR
Special Elements WPRE, cPPT
Protein Tag V5 Epitope Tag
Cloning Method Gateway
Quantity 20 Reactions
Vector pLenti
Product Line Gateway, HiPerform, T-REx, ViraPower
For Use With (Application) Viral Expression
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.