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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide

Product Code. 12060186
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Quantity:
800 x 20 μL Assays
Unit Size:
800 reactions
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Product Code. Quantity unitSize
12060186 800 x 20 μL Assays 800 reactions
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Product Code. 12060186 Supplier Invitrogen™ Supplier No. PV3793

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Beskrivelse

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
TRUSTED_SUSTAINABILITY

Tekniske data

Product Line Z´-LYTE
Target Serine/Threonine Kinases
For Use With (Application) FRET
Assay Serine/Threonine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Sample Type myosin light chain 2 (KKRPQRRYSNVF)
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
References JBC(2001) 276, p38956
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4
Validated Kinases CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
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For Research Use Only. Not for use in diagnostic procedures.

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