Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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Gibco™ PBS, pH 7.4

Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.3 to 7.5
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°C to 30°C
Concentration	1 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 - 315 mOsm/kg
Sterility	Sterile-filtered

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets

Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.

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Gibco™ DPBS, no calcium, no magnesium

DPBS, no calcium, no magnesium

Pricing and Availability
Specifications

Physical Form	Liquid
Chemical Name or Material	DPBS
Recommended Storage	15°to 30°C
Concentration	1 X

Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4

Non-toxic to most cells and is used in biological research.

Pricing and Availability
Specifications

Color	Colorless
Physical Form	Liquid
pH	7.4
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Ambient temperatures
Concentration	10 X

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Gibco™ PBS (10X), pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Color	White
Physical Form	Liquid
pH	7.4
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Storage conditions: 15°C to 30°C, Shipping conditions: Room temperature, Shelf life: 24 months from date of manufacture
Concentration	10X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3100 mOsm/kg
Sterility	Sterile
Solution Type	pH Buffer, Storage Solution and Cleaning Solution
Product Line	Gibco

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PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

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Gibco™ PBS (10X), pH 7.2

PBS (10X), pH 7.2

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.1 to 7.3
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	15°to 30°C
Concentration	10 X
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3200 mOsm/kg

Corning™ Cell Culture Phosphate Buffered Saline (1X)

With low endotoxin content. Without calcium and magnesium.

Pricing and Availability
Specifications

Formulation	Potassium Dihydrogen Phosphate 0.144g/L, Sodium Chloride 9g/L, Disodium Phosphate 0.795g/L
Physical Form	Liquid
pH	7.3 to 7.5
Recommended Storage	2° to 30°C
Concentration	1 X

Gibco™ HEPES (1M)

HEPES (1M)

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Gibco™ DPBS, no calcium, no magnesium

Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Pricing and Availability
Specifications

Physical Form	Liquid
pH	7.0 to 7.3
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	DPBS
For Use With (Application)	Mammalian Cell Culture
Osmolality	270 to 300 mOsm/kg
Sterility	Sterile
Solution Type	Cleaning, Storage, pH Buffer

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Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Pricing and Availability
Specifications

Formulation	50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide
Physical Form	Liquid
pH	7.7
Chemical Name or Material	Running Buffer
Recommended Storage	Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C
Concentration	20X
Product Line	NuPAGE

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Includes	No Calcium, No Magnesium
Color	Clear
Packaging	Bottle
Physical Form	Liquid
pH	7.4
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Concentration	1X
For Use With (Application)	Washing and Staining Cells
Osmolality	280 to 315 mOsm/kg
Sterility	Sterile
Solution Type	pH Buffer, Storage Solution and Cleaning Solution
Product Line	Gibco

Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)

With low endotoxin content

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Gibco™ HBSS, calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing and Availability
Specifications

Includes	No Sodium Pyruvate
Physical Form	Liquid
pH	6.7 to 7.8
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Chemical Name or Material	Hanks' Balanced Salt Solution (HBSS)
Concentration	1X
For Use With (Application)	Mammalian Cell Culture
Osmolality	270 to 305 mOsm/kg
Sterility	Sterile
Solution Type	pH Buffer, Storage Solution and Cleaning Solution
Product Line	Gibco

Life Science Buffers

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Corning™ Cell Culture Phosphate Buffered Saline (1X) SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X) SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Corning™ Cell Culture Phosphate Buffered Saline (1X)

Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)