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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (4,001)
HPLC Guard Columns and Cartridges (337)
HPLC Guard Column Accessories (43)

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1 – 15 of 1,029 results
1
Promotions Available

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3
Promotions Available

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
4

Thermo Scientific™ Uniguard™ Direct-Connection Guard Cartridge Holders

Eliminate the requirement for extra fittings with Thermo Scientific™ Uniguard Direct-Connection Guard Cartridge Holders.

EDGE
Type Cartridge Holder
5
Promotions Available

Thermo Scientific™ Hypersil™ APS-2 HPLC Columns, 5μm Particle Size
BIOPHARMA

Analyze sugars with this versatile amino propyl phase of Thermo ScientificTM HypersilTM APS-2 HPLC Columns.

EDGE
Pore Size 120 Å
Carbon Load 1.9%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Packing Material Spherical, Fully Porous Type A Silica
Column Type Carbohydrate Analysis
pH 2.5 to 8
Surface Area 170 m²/g
USP Type L8
6

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
BIOPHARMA

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

EDGE
7
Promotions Available

Thermo Scientific™ EASY-Spray™ HPLC Columns
BIOPHARMA

Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.

EDGE
Phase Reversed Phase
8

Thermo Scientific™ POROS™ G 20 μm Protein G Affinty HPLC Columns

Thermo Scientific™ POROS™ Protein G is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.

EDGE
Particle Size 20 μm
Stationary Phase Recombinant Protein G
Binding Capacity 9 mg/mL
Column Type Affinity
Material PEEK
Product Line POROS
9

Thermo Scientific™ Accucore™ aQ C18 Polar Endcapped HPLC Columns

Use this column, with its specially designed solid core, for the separation of compounds in highly aqueous mobile phases.

EDGE
Pore Size 80 Å
Stationary Phase aQ C18
Temperature 70°C
Phase Reversed Phase
Endcapped Polar
Surface Area 130 m²/g
Max. Pressure 14500 psi (1000 bar)
Particle Size 2.6 μm
Carbon Load 9%
Packing Material Ultrapure Silica
pH 2 to 9
Particle Shape Solid Core
Product Line Accucore
USP Type L1
10
Promotions Available

Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Columns
BIOPHARMA

Separate challenging peptide mapping samples with Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns.

EDGE
Pore Size 100 Å
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
For Use With (Application) Bottom-up Proteomics, LC-MS
Surface Area 450 m2/g
Max. Pressure 22,000 psi (1500 bar)
Column Format Analytical Column, EASY-Spray™
Particle Size 2 μm
Carbon Load 15%
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 75 μm
pH 2 to 8
Product Line Easy Spray
USP Type L1
11

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
BIOPHARMA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
12

Thermo Scientific™ POROS™ CaptureSelect™ CH1-XL Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ CH1-XL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands.

EDGE
Shipping Condition Wet Ice
Pore Size 2000 Å
Content And Storage Column includes EZ grip fittings and column test certificate.

Store at 2°C to 8°C. DO NOT FREEZE.
Stationary Phase IgG Fab affinity ligand, CH1 domain of human IgG antibodies
Temperature 2-40°C
Column Type Affinity
Quantity 1 Column
Material PEEK
Max. Pressure 2400 psi (170 bar)
Column Format Analytical Column, Biocompatible PEEK
Particle Size 20 μm
Packing Material Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Binding Capacity 26.2 mg/ml human IgG 8.8 mg/ml human Fab fragment
pH 1-10
Technique Bio-LC
Product Line POROS CaptureSelect
13

Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

EDGE
Shipping Condition Wet Ice
Pore Size 2000 Å
Content And Storage Column includes EZ grip fittings and column test certificate

Store at 4°C. DO NOT FREEZE.
Stationary Phase Human Serum Albumin affinity ligand, humanspecific
Column Type Affinity
Quantity 1 Column
Material PEEK
High-throughput Compatibility Not High-throughput Compatible (Manual)
Max. Pressure 180 bar
Particle Size 20 μm
Flow Rate Up to 5000 cm/hr.
Type Column
Matrix Polystyrenedivinylbenzene
Reactivity Human
Target Molecule HSA, HSA Fusion Proteins
Binding Capacity 2.6 mg/mL
Sample Type Cell Culture Supernatants
Purification Target HSA, HSA Fusion Proteins
Product Line CaptureSelect, POROS
14

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

EDGE
Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
15

Thermo Scientific™ Syncronis™ HILIC HPLC Columns

Maximize liquid chromatography reproducibility and laboratory productivity using Thermo Scientific™ Syncronis™ HILIC LC Columns.

EDGE
Pore Size 100 Å
Carbon Load 5%
Stationary Phase Zwitterionic
Temperature 60°C
Column Type HILIC
pH 2 to 8
Surface Area 320 m²/g