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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 3,942 results
1

Thermo Scientific™ Acclaim™ 120 C8 HPLC Columns

Achieve exceptional performance and maximum resolution for reversed-phase separation of analytes with moderate hydrophobicity.

Pore Size 120 Å
Carbon Load 11%
Stationary Phase C8
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L7
2
Promotions Available

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3
Promotions Available

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
4
Promotions Available

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
BIOPHARMA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
5
Promotions Available

Thermo Scientific™ Accucore™ C18 HPLC Columns
BIOPHARMA

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

EDGE
Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 9%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 130 m²/g
Particle Shape Solid Core
USP Type L1
6
Promotions Available

Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon HPLC Columns
BIOPHARMA

Use for enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

EDGE
7
Promotions Available

Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC Columns

Obtain exceptional peak shape and high resolution for fast UHPLC high-pressure, high-throughput applications using generic gradients on Vanquish UHPLC and LC/MS systems.

EDGE
Pore Size 175 Å
Stationary Phase C18 Selectivity
Temperature 60°C
Phase Reversed Phase
For Use With UHPLC
Endcapped Yes
Surface Area 220 m²/g
Column Format Analytical Column
Particle Size 1.9 μm
Carbon Load 10%
Packing Material Ultrapure Silica
Diameter (Metric) 2.1 mm
pH 1 to 11
Product Line Hypersil GOLD
USP Type L1
8

Thermo Scientific™ POROS™ G 20 μm Protein G Affinty HPLC Columns

Thermo Scientific™ POROS™ Protein G is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.

EDGE
Particle Size 20 μm
Stationary Phase Recombinant Protein G
Binding Capacity 9 mg/mL
Column Type Affinity
Material PEEK
Product Line POROS
9
Promotions Available

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

EDGE
Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
10
Promotions Available

Thermo Scientific™ Acclaim™ 120 C18 Columns

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

EDGE
Pore Size 120 Å
Carbon Load 18%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L1
11
Promotions Available

Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 HPLC Columns

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns.

EDGE
Pore Size 120 Å
Phase Reversed Phase, Ion Exchange, HILIC
Column Type Mixed Mode
pH 2.5 to 7.5
Surface Area 300 m²/g
Product Line Acclaim
USP Type L78
12
Promotions Available

Thermo Scientific™ Hypersil™ APS-2 HPLC Columns, 5μm Particle Size
BIOPHARMA

Analyze sugars with this versatile amino propyl phase of Thermo ScientificTM HypersilTM APS-2 HPLC Columns.

EDGE
Pore Size 120 Å
Carbon Load 1.9%
Stationary Phase Amino (NH2)
Temperature 60°C
Phase Reversed Phase, Normal Phase, Ion Exchange, HILIC
Endcapped No
Packing Material Spherical, Fully Porous Type A Silica
Column Type Carbohydrate Analysis
pH 2.5 to 8
Surface Area 170 m²/g
USP Type L8
13
Promotions Available

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
BIOPHARMA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
14
Promotions Available

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SCX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and biosimilar proteins.

EDGE
Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Proprietary
Temperature Ambient to 60°C
Phase Strong Cation Exchange
Packing Material Monodisperse Polymer Resin
Column Type Ion Exchange
Mobile Phase Compatibility Recommended for use with CX-1 pH buffers
pH 2 to 12
Technique Bio-LC
Max. Pressure 4500 psi (310 bar)
Product Line ProPac
Column Format Analytical Column
15

Thermo Scientific™ SurePac™ Protein RP MDi™ HPLC Columns

SurePac Protein RP MDi is a bioinert reversed-phase column engineered for robust HPLC and LC-MS analysis of intact proteins, mAbs and ADCs. It minimizes unspecific interactions with the column hardware, improves recovery, and delivers reproducible, high-resolution separations for sensitive therapeutic proteins workflows.

EDGE