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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (14,811)
ELISA Substrates and Reagents (1,237)
Singleplex and Multiplex Bead Based Immunoassays (1,039)

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1 – 15 of 14,143 results
1
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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

EDGE
2

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

EDGE
3
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Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

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4
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Invitrogen™ Human IFN gamma ELISA Kit

ELISA

EDGE
Measurement Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format Sandwich ELISA Kit
Target Species Human
Conjugate HRP
Description Human IFN gamma ELISA Kit
Interassay CV <12%
Intraassay CV <10%
Gene Symbol IFNG
Total Assay Time 3 hr
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <2 pg/mL
Storage Requirements 2-8°C
Assay Range 25.6-1000 pg/mL
Test Time 1 hr 20 min
Accession Number P01579
Regulatory Status RUO
Target IFN gamma
Specificity No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3458
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
5
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Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

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6
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Invitrogen™ ELISA Buffer Kit

Concentrated

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7

Human IL-6 ELISA Development Kit (TMB), PeproTech®

ELISA

EDGE
8

Invitrogen™ Human MIG/CXCL9 ELISA Kit

ELISA

EDGE
Protein Family GPCR (G-Protein Coupled Receptors)
Target Species Human
Conjugate HRP
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 20 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Target CXCL9 (MIG)
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Label or Dye Biotin
Calibration Range 20 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol CXCL9
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range 20-6000 pg/mL
Accession Number Q07325
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4283
Sample Type Plasma,Serum,Supernatant
Synonym CMK, Humig, MIG, SCYB9, crg-10
9

Invitrogen™ Human IL-6 ELISA Kit, High Sensitivity

ELISA

EDGE
10
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Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

EDGE
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Product Line CDP-Star, NovaBright
11
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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
12

Invitrogen™ Human IL1RA ELISA Kit

ELISA

EDGE
13

Invitrogen™ Human CA19-9 ELISA Kit

ELISA

EDGE
Protein Family Other Proteins
Target Species Human
Conjugate HRP
Calibration Range 0.3 U/mL to 200 U/mL
Interassay CV <12%
Intraassay CV <10%
Total Assay Time 4 hr 45 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 0.3 U/mL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Research Discipline Other Research
Assay Range 0.3-200 U/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hr 20 min
Assay Solid-phase sandwich ELISA
Regulatory Status RUO
Target CA19-9
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP- 2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
14

Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

EDGE
Target Species Mouse
Conjugate HRP
Interassay CV 6.7%
Intraassay CV 5.8%
Gene Symbol Il6
Total Assay Time 3 hr 30 min
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity <3 pg/mL
Storage Requirements 2-8°C
Assay Range 7.8-500 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hr 20 min
Accession Number P08505
Regulatory Status RUO
Target IL-6
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <3 pg/mL
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 16193
Sample Type Plasma,Serum,Supernatant
Label or Dye Biotin
15

Invitrogen™ Human Tau (Total) ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.5%
Intraassay CV 4.6%
Incubation Time 4 hr.
Gene Symbol MAPT
Total Assay Time 4 hr
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL
Assay Sensitivity <10 pg/mL
Storage Requirements 2-8°C
Concentration 31 to 2000 pg/mL
Assay Range 31-2000 pg/mL
Marker Gene Tau
Test Time 1 hr 20 min
Accession Number P10636
Regulatory Status RUO
Target Tau
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4137
Sample Type Cerebrospinal Fluid,Supernatant
Label or Dye Biotin