Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Thermo Scientific™ PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
| Substrate Properties | Chemical Substrate |
|---|---|
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Detection Method | Colorimetric |
Invitrogen™ TMB ELISA Buffer Kit, PeproTech®
This kit contains the key components required for the analysis of 10 Sandwich ELISA plates. It has been specifically formulated to be used in conjunction with PeproTech’s Standard TMB ELISA Development Kits.
Thermo Scientific™ SuperSignal™ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
| Content And Storage | Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature. |
|---|---|
| Substrate Properties | Chemical Substrate |
| Product Type | ELISA Substrate |
| Substrate Type | HRP (Horseradish Peroxidase) |
| Product Line | SuperSignal™ |
| Detection Method | Chemiluminescence |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.5% |
| Intraassay CV | 4.6 |
| Incubation Time | 4 hr. |
| Gene Symbol | DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT |
| Total Assay Time | 4 hrs |
| Sample Volume | Cerebrospinal Fluid 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <10 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Concentration | 31 to 2000 pg/mL |
| Assay Range | 31 to 2000 pg/mL |
| Marker Gene | Tau |
| Test Time | 1 hrs 20 mins |
| Accession Number | P10636 |
| Regulatory Status | RUO |
| Target | Tau |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4137 |
| Sample Type | Cerebrospinal Fluid,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Tissue Extraction Reagent I
Lysis buffer for total protein extraction from tissue samples
| Measurement | Absorbance at 450 nm minus absorbance (at 550 nm) |
|---|---|
| Immunoassay Kit Format | Colorimetric ELISA (TMB substrate) with Plates |
| Target Species | Human |
| Conjugate | HRP |
| Description | Human IFN gamma ELISA Kit |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFG,IFI |
| Total Assay Time | 3 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <2 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 25.6 to 1000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | P01579 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | Intra-assay CV ≤ 10%; inter-assay CV ≤ 10% |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3458 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.1% |
| Intraassay CV | 3.9 |
| Gene Symbol | GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1 |
| Total Assay Time | 2 hrs 30 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <5 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 15.6 to 1000 pg/mL |
| For Use With (Application) | Colorimetric |
| Test Time | 1 hrs 20 mins |
| Accession Number | P10145 |
| Regulatory Status | RUO |
| Target | IL-8 |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | <5 pg/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3576 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 8.1% |
| Intraassay CV | 4.7 |
| Incubation Time | 4 hr. |
| Gene Symbol | MVCD1,VEGF,VPF |
| Total Assay Time | 4 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <5 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 23.4 to 1500 pg/mL |
| Marker Gene | VEGF |
| For Use With (Application) | Colorimetric Detection |
| Test Time | 1 hrs 20 mins |
| Accession Number | P15692 |
| Regulatory Status | RUO |
| Target | VEGF |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 7422 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 5.6% |
| Intraassay CV | 3.3 |
| Incubation Time | 4 hr. |
| Gene Symbol | CLG4,CLG4A,MMP-2,MMP-II,MONA,TBE-1 |
| Total Assay Time | 4 hrs |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <1 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.78 to 50 ng/mL |
| Marker Gene | MMP-2 |
| For Use With (Application) | Colorimetric Detection |
| Test Time | 1 hrs 20 mins |
| Accession Number | P08253 |
| Regulatory Status | RUO |
| Target | MMP2 |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4313 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.7% |
| Intraassay CV | 5.8 |
| Gene Symbol | IL-6 |
| Total Assay Time | 3 hrs 30 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | <3 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 7.8 to 500 pg/mL |
| For Use With (Application) | Colorimetric |
| Test Time | 1 hrs 20 mins |
| Accession Number | P08505 |
| Regulatory Status | RUO |
| Target | IL-6 |
| Kit Contents | Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Precision | <3 pg/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 16193 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
Invitrogen™ Cytokine 30-Plex Human Panel
Specifically designed for quantifying human cytokines, chemokines and growth factors in serum, plasma, and tissue culture supernatant
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | LBP |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.82 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.82 to 200 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | acute phase protein; binds bacterial LPS; BPI fold containing family D, member 2; Bpifd2; hypothetical protein LOC512242; Lbp; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; lipopolysaccharide-binding protein LPSBP; LPS-binding protein; LPSBP; Ly88 |
| Regulatory Status | RUO |
| Target | LBP |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 3929 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |