Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Invitrogen™ TMB Chromogen Solution (for ELISA)
May be used as a substrate for horseradish peroxidase (HRP) in ELISAs
Invitrogen™ Glutathione Colorimetric Detection Kit
Assay Glutathione levels in Whole Blood, Serum, Plasma, Erythrocytes, Urine, Cell Lysates and Tissue Samples (colorimetric).
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Colorimetric Detection Kit |
| Content And Storage | 96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C. |
| For Use With (Equipment) | Microplate Reader |
| Product Type | Glutathione Colorimetric Detection Kit |
| Gene ID (Entrez) | Non-Gene |
| Sample Type | Other Biological Fluids,Plasma,Serum |
| Gene Symbol | Non-Gene |
| Target Kit Named | Glutathione |
| Detection Method | Colorimetric |
Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Invitrogen™ Tissue Extraction Reagent I
Lysis buffer for total protein extraction from tissue samples
| Shipping Condition | Wet Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human,Mouse,Rat |
| Conjugate | HRP |
| Interassay CV | 4% |
| Intraassay CV | 3.7% |
| Gene Symbol | NEFL |
| Total Assay Time | 3 hr 45 min |
| Assay Sensitivity | 0.8 pg/mL |
| Storage Requirements | 2-8°C |
| Assay Range | 1.6-1200 |
| Test Time | 1 hr 20 min |
| Accession Number | P07196, P08551, P19527 |
| Regulatory Status | RUO |
| Target | NF-L |
| Kit Contents | Pre-coated microwell plate, Standard, Biotinylated Detection Antibody, Streptavidin-HRP, Sample Diluent, Wash Buffer Concentrate, Assay Buffer concentrate, Stop Solution, Substrate Solution, Adhesive Plate Covers |
| Gene Alias | 68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L; |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 18039, 4747, 83613 |
| Sample Type | Cerebrospinal Fluid,Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
| Protein Family | Other Proteins |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 2 μL, Serum 2 μL, Supernatant 100 μL |
| Assay Sensitivity | 0.13 ng/mL |
| Research Discipline | Angiogenesis |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | VWF |
| Specificity | This ELISA antibody pair detects human vWF. Other species not determined. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 0.4 ng/mL to 120 ng/mL |
| Interassay CV | <12% |
| Gene Symbol | VWF |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.123-30 ng/mL |
| Accession Number | P04275 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 7450 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | F8VWF, VWD |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Interassay CV | 6.5% |
| Intraassay CV | 4.6% |
| Incubation Time | 4 hr. |
| Gene Symbol | MAPT |
| Total Assay Time | 4 hr |
| Sample Volume | Cerebrospinal Fluid 50 μL, Supernatant 50 μL |
| Assay Sensitivity | <10 pg/mL |
| Storage Requirements | 2-8°C |
| Concentration | 31 to 2000 pg/mL |
| Assay Range | 31-2000 pg/mL |
| Marker Gene | Tau |
| Test Time | 1 hr 20 min |
| Accession Number | P10636 |
| Regulatory Status | RUO |
| Target | Tau |
| Kit Contents | Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 4137 |
| Sample Type | Cerebrospinal Fluid,Supernatant |
| Label or Dye | Biotin |
| Target Species | Human |
|---|---|
| Conjugate | FAM |
| Gene Symbol | Il6 |
| Total Assay Time | 2 hr |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.05 pg/mL |
| Storage Requirements | -20°C |
| Assay Range | 0.064-10000 pg/mL |
| For Use With (Application) | Cytokine Protein Quantitation |
| Test Time | 30 min |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P05231 |
| Regulatory Status | RUO |
| Target | IL-6 |
| Kit Contents | Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 3569 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
| Protein Family | GPCR (G-Protein Coupled Receptors) |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 20 pg/mL |
| Research Discipline | Inflammation |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | CXCL9 (MIG) |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 20 pg/mL to 6000 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | CXCL9 |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 20-6000 pg/mL |
| Accession Number | Q07325 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 4283 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | CMK, Humig, MIG, SCYB9, crg-10 |
| Protein Family | Cell Structure/Organelle Proteins |
|---|---|
| Target Species | Human |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 1 μL, Serum 1 μL, Supernatant 100 μL |
| Assay Sensitivity | 0.08 pg/mL |
| Research Discipline | Extracellular Matrix & Cell Adhesion |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | D-dimer |
| Specificity | This ELISA pair antibody detects human D-Dimer. Other species not determined. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 0.08 pg/mL to 60 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | FGA |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.082-60 pg/mL |
| Accession Number | P02671 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 2243 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | Fib2 |
Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)
The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
| Content And Storage | Store the product at 4°C. |
|---|---|
| Format | Bottle |
| Form | Liquid |
| Product Type | ELISA Substrate |
| Substrate Type | Alkaline Phosphatase |
| Product Line | CDP-Star, NovaBright |