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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (14,592)
ELISA Substrates and Reagents (1,231)
Singleplex and Multiplex Bead Based Immunoassays (1,019)

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1
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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAVX Affinity Matrix.

EDGE
Shipping Condition Dry Ice
Product Line CaptureSelect
3
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Invitrogen™ Human IL-8 ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.1%
Intraassay CV 3.9
Gene Symbol GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time 2 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity <5 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 15.6 to 1000 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P10145
Regulatory Status RUO
Target IL-8
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <5 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3576
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
4

Invitrogen™ ELISA Buffer Kit

Concentrated

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5

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

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Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
6

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

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7

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

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8

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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9
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Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

EDGE
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
10
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Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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11
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Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

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12
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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

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Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
13

Invitrogen™ Human MIG/CXCL9 ELISA Kit

ELISA

EDGE
Protein Family GCPR
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 20 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CXCL9 (MIG)
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 20 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol CMK,Humig,MIG,SCYB9,crg-10
Storage Requirements 2°C to 8°C
Assay Range 8.23 to 6000 pg/mL
Accession Number Q07325
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4283
Sample Type Plasma,Serum,Supernatant
Synonym CMK, Humig, MIG, SCYB9, crg-10
14
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Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

EDGE
Target Species Mouse
Conjugate HRP
Interassay CV 6.7%
Intraassay CV 5.8
Gene Symbol IL-6
Total Assay Time 3 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity <3 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 7.8 to 500 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P08505
Regulatory Status RUO
Target IL-6
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <3 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 16193
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15

Invitrogen™ Human Tau (Total) ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.5%
Intraassay CV 4.6
Incubation Time 4 hr.
Gene Symbol DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT
Total Assay Time 4 hrs
Sample Volume Cerebrospinal Fluid 50 μL, Supernatant 50 μL
Assay Sensitivity <10 pg/mL
Storage Requirements 2°C to 8°C
Concentration 31 to 2000 pg/mL
Assay Range 31 to 2000 pg/mL
Marker Gene Tau
Test Time 1 hrs 20 mins
Accession Number P10636
Regulatory Status RUO
Target Tau
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 4137
Sample Type Cerebrospinal Fluid,Supernatant
Label or Dye HRP