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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (14,631)
ELISA Substrates and Reagents (1,239)
Singleplex and Multiplex Bead Based Immunoassays (1,019)

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1 – 15 of 14,017 results
1

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

EDGE
2
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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

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3
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Invitrogen™ ELISA Buffer Kit

Concentrated

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4
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Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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5

Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ CH1-XL Ligand Leakage ELISA Kit is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect CH1-XL Affinity Matrix.

EDGE
Shipping Condition Dry Ice
Content And Storage Store in freezer (-5°C to -30°C).
Format Kit
6
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Invitrogen™ Tissue Extraction Reagent I

Lysis buffer for total protein extraction from tissue samples

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7

Invitrogen™ Malondialdehyde (MDA) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

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8
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Invitrogen™ Human IL-1 beta ELISA Kit

ELISA

EDGE
Target Species Human
Conjugate HRP
Interassay CV 6.6%
Intraassay CV 4.4
Gene Symbol IL-1,IL1-BETA,IL1F2
Total Assay Time 2 hrs 55 mins
Sample Volume Serum 50 μL, Supernatant 50 μL
Assay Sensitivity 1 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 3.9 to 250 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P01584
Regulatory Status RUO
Target IL-1 beta
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision 1 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 3553
Sample Type Serum,Supernatant
Label or Dye HRP
9
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Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

EDGE
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Product Line CDP-Star, NovaBright
10
Promotions Available

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

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Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
11

Invitrogen™ Porcine IL-6 ELISA Kit

ELISA

EDGE
Protein Family Cytokines & Receptors
Conjugate HRP
Interassay CV <12%
Intraassay CV <10
Gene Symbol IL6
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 45 pg/mL
Storage Requirements 2°C to 8°C
Research Discipline Immunology
Assay Range 40.96 to 10,000 pg/mL
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Detection Method Colorimetric
Assay Solid-phase sandwich ELISA
Accession Number P26893
Regulatory Status RUO
Target IL-6
Specificity The sandwich ELISA antibody pair detects Porcine IL-6.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 399500
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
12

Invitrogen™ Human IL1RA ELISA Kit

ELISA

EDGE
13

Invitrogen™ Human MIG/CXCL9 ELISA Kit

ELISA

EDGE
Protein Family GCPR
Target Species Human
Conjugate HRP
Intraassay CV <10
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity 20 pg/mL
Research Discipline Inflammation
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Assay Solid-phase sandwich ELISA
Target CXCL9 (MIG)
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 20 pg/mL to 6000 pg/mL
Interassay CV <12%
Gene Symbol CMK,Humig,MIG,SCYB9,crg-10
Storage Requirements 2°C to 8°C
Assay Range 8.23 to 6000 pg/mL
Accession Number Q07325
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 4283
Sample Type Plasma,Serum,Supernatant
Synonym CMK, Humig, MIG, SCYB9, crg-10
14

Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

EDGE
Target Species Mouse
Conjugate HRP
Interassay CV 6.7%
Intraassay CV 5.8
Gene Symbol IL-6
Total Assay Time 3 hrs 30 mins
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity <3 pg/mL
Storage Requirements 2°C to 8°C
Assay Range 7.8 to 500 pg/mL
For Use With (Application) Colorimetric
Test Time 1 hrs 20 mins
Accession Number P08505
Regulatory Status RUO
Target IL-6
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision <3 pg/mL
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Gene ID (Entrez) 16193
Sample Type Plasma,Serum,Supernatant
Label or Dye HRP
15

Invitrogen™ Human IL-6 ProQuantum Immunoassay Kit
GREENER_CHOICE

ELISA

EDGE
Target Species Human
Conjugate FAM™
Gene Symbol BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.05 pg/mL
Storage Requirements -20°C
Assay Range 0.064 to 10000 pg/mL
For Use With (Application) Cytokine Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P05231
Regulatory Status RUO
Target IL-6
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3569
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™