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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (918)
Standard PCR Products (509)
Reverse Transcription Products (111)
dNTPs (59)
DNA Polymerases (54)

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Promotions Available

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
2
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Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
3
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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type High-Fidelity DNA Polymerase
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Standard PCR,High-fidelity PCR
4
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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
5
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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE
Shipping Condition Wet Ice
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Medium
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
6
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Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

EDGE
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
7
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Thermo Scientific™ dNTP Set, 100 mM Solutions

Thermo Scientific™ dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 100 mM
For Use With (Application) Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)
8
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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

EDGE
Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 120 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Quantity 1 Ea.
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA
9
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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
10
Promotions Available

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
11
Promotions Available

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR
Detection Method SYBR
12
Promotions Available

Invitrogen™ Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

EDGE
Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
13
Promotions Available

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
14
Promotions Available

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Kit
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
15
Promotions Available

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

EDGE
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan