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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1
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Gibco™ TrypLE™ Express Enzyme (1X), phenol red
GREENER_CHOICE

Animal origin-free, recombinant enzyme

EDGE
Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
2
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Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

EDGE
3

Gibco™ Trypsin (1:250), powder

Trypsin (1:250), powder

EDGE
4
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Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

EDGE
Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
5
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Gibco™ Trypsin-EDTA (0.05%), phenol red
GREENER_CHOICE

Trypsin-EDTA (0.05%), phenol red

EDGE
Regulatory Status RUO
Content And Storage –5 to –20°C
With Additives EDTA,Phenol Red
Product Type Cell Culture Dissociation Reagent
Form Liquid
Shelf Life 24 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
Source Porcine pancreas
Cell Type Mammalian Cells
6
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Gibco™ Cell Dissociation Buffer, enzyme-free, PBS

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

EDGE
7

Gibco™ Trypsin (0.25%), phenol red
GREENER_CHOICE

Trypsin (0.25%), phenol red

EDGE
Regulatory Status RUO
Content And Storage –5 to –20°C
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No EDTA
Shelf Life 18 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
Source Porcine pancreas
Cell Type Mammalian Cells
8

Gibco™ Trypsin Neutralizer Solution
GREENER_CHOICE

Sterile, phosphate-buffered saline solution

EDGE
9
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Gibco™ Trypsin-EDTA (0.5%), no phenol red
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

10
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Gibco™ TrypLE™ Select Enzyme (1X), no phenol red
GREENER_CHOICE

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Phenol Red
Shelf Life 24 Months
Concentration 1 X
11
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Corning™ Accutase™ Cell Detachment Solution, Liquid
BIOPHARMA

Efficient, with gentle detachment of adherent cells within minutes

Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
12

Gibco™ Defined Trypsin Inhibitor
GREENER_CHOICE

Animal origin-free

13
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Gibco™ Collagenase, Type IV, powder

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

14
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Gibco™ Collagenase, Type I, powder

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
15
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Gibco™ Collagenase, Type II, powder

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen