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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.

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1
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Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

EDGE
2
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Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware.

3
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Gibco™ Cell Dissociation Buffer, enzyme-free, PBS

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

4

BD Cell Detachment Solution

Replacement for trypsin solution and can be used for the passaging of cell lines

5
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Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C
6
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Corning™ Accutase™ Cell Detachment Solution, Liquid
BIOPHARMA

Efficient, with gentle detachment of adherent cells within minutes

Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
7
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Gibco™ Trypsin-EDTA (0.05%), phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

EDGE
Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
8

STEMCELL Technologies Animal Component-Free Cell Dissociation Kit

Dissociation kit for human mesenchymal stem cells

ENCOMPASS_PREFERRED
9
Promotions Available

Gibco™ TrypLE™ Express Enzyme (1X), phenol red

Animal origin-free, recombinant enzyme

EDGE
Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
10
Promotions Available

Gibco™ Trypsin-EDTA (0.25%), phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

EDGE
Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
11

Gibco™ Trypsin (1:250), powder

Trypsin (1:250), powder

EDGE
12

Gibco™ Defined Trypsin Inhibitor

Animal origin-free

EDGE
13

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium
BIOPHARMA

Used to enzymatically release adherent cells from tissue culture plates for passaging.

EDGE
14
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Gibco™ Versene Solution

An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent

EDGE
15
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Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

EDGE
Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco