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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.

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1

Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

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2
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Gibco™ Cell Dissociation Buffer, enzyme-free, PBS

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

3
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Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C
4

BD Cell Detachment Solution

Replacement for trypsin solution and can be used for the passaging of cell lines

5
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Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware.

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Gibco™ TrypLE™ Express Enzyme (1X), phenol red
GREENER_CHOICE

Animal origin-free, recombinant enzyme

EDGE
Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
7
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Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
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TrypLE™ Select Enzyme (10X), no phenol red

EDGE
Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
8
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Gibco™ Trypsin-EDTA (0.05%), phenol red
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Trypsin-EDTA (0.05%), phenol red

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Regulatory Status For research use only
Content And Storage –5 to –20°C
With Additives EDTA,Phenol Red
Product Type Cell Culture Dissociation Reagent
Form Liquid
Shelf Life 24 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
Source Porcine pancreas
Cell Type Mammalian Cells
9

Gibco™ Trypsin (0.25%), phenol red
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Trypsin (0.25%), phenol red

EDGE
Regulatory Status For research use only
Content And Storage –5 to –20°C
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No EDTA
Shelf Life 18 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
Source Porcine pancreas
Cell Type Mammalian Cells
10
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Gibco™ Trypsin-EDTA (0.05%), phenol red
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
11
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Gibco™ Versene Solution
GREENER_CHOICE

An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent

12
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Gibco™ TrypLE™ Express Enzyme (1X), no phenol red
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
13
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Gibco™ TrypLE™ Select Enzyme (1X), no phenol red
GREENER_CHOICE

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Phenol Red
Shelf Life 24 Months
Concentration 1 X
14

Gibco™ Versene Solution
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Versene Solution

15
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Gibco™ CTS™ TrypLE™ Select Enzyme
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TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation.