| Content And Storage |
Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water
Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid
Store at -68 to -85°C. |