Modifying Enzymes
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Invitrogen™ Ambion™ RNase A, affinity purified, 1 mg/mL
Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity
Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Invitrogen™ Ambion™ DNase I (RNase-free)
Nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Invitrogen™ TrueCut™ Cas9 Protein v2
A next-generation CRISPR Cas9 protein engineered to deliver maximum on-target editing efficiency. TrueCut Cas9 Protein v2 is ideal for applications requiring the highest editing efficiency.
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Pepsin, Extra Pure, Powder, SLR, Fisher Chemical™
Enzyme that breaks down proteins into smaller peptides.
Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Invitrogen™ M13KO7 Helper Phage
Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication
Thermo Scientific Chemicals Pectinase, Aspergillus niger
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.