Miscellaneous Molecular Biology Reagents
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ IPTG, dioxane-free
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ RNA Gel Loading Dye (2X)
Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Polysorbate 20
CAS: 9005-64-5 Molecular Formula: C26H50O10 Synonym: Polyoxyethylene(20)sorbitan monolaurate
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Invitrogen™ Linear Acrylamide (5 mg/ml) (1 ml Tube)
Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.
Thermo Scientific™ MassRuler DNA Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.
Invitrogen™ SSC (20X), RNase-free
Molecular biology grade, Invitrogen 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Thermo Scientific™ Yeast DNA Extraction Kit
Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.
Invitrogen™ IPTG (Dioxane-Free)
Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.