Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Invitrogen™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Pierce™ Cell Surface Biotinylation and Isolation Kit
The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.
Invitrogen™ Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Fluorescein-5-Isothiocyanate (FITC 'Isomer I')
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.
Thermo Scientific™ Proteinase K Powder (≥30 U/mg)
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Molecular Probes™ Alexa Fluor™ 647 Azide, Triethylammonium Salt
The far red-fluorescent Alexa Fluor™ 647 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
Invitrogen™ Alexa Fluor™ 647 C2 Maleimide
Alexa Fluor™ 647 is a bright, far-red fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines.
Invitrogen™ Click-iT™ Plus OPP Alexa Fluor™ 647 Protein Synthesis Assay Kit
Provides non-radioactive method for detection of protein synthesis using fluorescence microscopy or high-content imaging